Expression of molecular markers detected by immunohistochemistry and risk of lymph node metastasis in stage T1 and T2 colorecrectal cancers.
- VernacularTitle:T1和T2期结直肠癌淋巴结转移与相关分子标记物表达的关系
- Author:
Fu-long WANG
1
;
De-sen WAN
;
Zhen-hai LU
;
Yu-jing FANG
;
Li-ren LI
;
Gong CHEN
;
Xiao-jun WU
;
Pei-rong DING
;
Ling-heng KONG
;
Jun-zhong LIN
;
Zhi-zhong PAN
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Biomarkers, Tumor; metabolism; Colonic Neoplasms; metabolism; pathology; Female; Humans; Hyaluronan Receptors; metabolism; Immunohistochemistry; Lymphatic Metastasis; Male; Microsatellite Instability; Middle Aged; Neoplasm Staging; Oligonucleotide Array Sequence Analysis; Receptor, ErbB-2; metabolism; Receptors, CXCR4; metabolism; Rectal Neoplasms; metabolism; pathology; Retrospective Studies; Tissue Inhibitor of Metalloproteinase-1; metabolism; Vascular Endothelial Growth Factor Receptor-3; metabolism
- From: Chinese Journal of Oncology 2013;35(4):277-281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the molecular risk factors of lymph node metastasis in stage T1 and T2 colorectal cancers by tissue microarray and immunohistochemistry techniques.
METHODSTwo hundred and three patients with stage T1 and T2 colorectal carcinoma who underwent radical surgery from 1999 to 2010 in our department were included in this study. Their clinicopathological data were retrospectively analyzed. Expression of the following 14 molecular markers were selected and assayed by tissue microarray and immunohistochemistry: VEGFR-3, HER2, CD44v6, CXCR4, TIMP-1, EGFR, IGF-1R, IGF-2, IGFBP-1, ECAD, MMP-9, RKIP, CD133, MSI. Chi-squared test and logistic regression were used to evaluate the variables as potential risk factors for lymph node metastasis.
RESULTSThe positive expression rates of biomarkers were as following: VEGFR-3 (44.3%), EGFR (30.5%), HER-2 (28.1%), IGF-1R (63.5%), IGF-2 (44.8%), IGFBP-1 (70.9%), ECAD (45.8%), CD44v6 (51.2%), MMP-9 (44.3%), TIMP-1 (41.4%), RKIP (45.3%), CXCR4 (40.9%), and CD133 (49.8%). The positive rate of MSI expression was 22.2%. Both univariate and multivariate analyses showed that VEGFR-3, HER-2, and TIMP-1 were significant predictors of lymph node metastasis. Univariate analysis showed that CD44v6 and CXCR4 were significant significant predictors of lymph node metastasis.
CONCLUSIONSVEGFR-3, HER2 and TIMP-1 are independent factors for lymph node metastasis in stage T1 and T2 colorectal cancers.
