Microdeletions in the Y chromosome of patients with idiopathic azoospermia.
- Author:
Akiyuki SHIMIZU
1
;
Tomohiko ICHIKAWA
;
Noriyuki SUZUKI
;
Takako YAMAZAKI
;
Takashi IMAMOTO
;
Satoko KOJIMA
;
Yukio NAYA
;
Akira KOMIYA
;
Hiroyoshi SUZUKI
;
Koichi NAGAO
;
Kazukiyo MIURA
;
Haruo ITO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Base Sequence; Chromosomes, Human, Y; DNA Primers; Euchromatin; genetics; Follicle Stimulating Hormone; blood; Heterochromatin; genetics; Humans; Luteinizing Hormone; blood; Male; Oligospermia; blood; etiology; genetics; Polymerase Chain Reaction; Prolactin; blood; Sequence Deletion; genetics; Sequence Tagged Sites; Testosterone; blood
- From: Asian Journal of Andrology 2002;4(2):111-115
- CountryChina
- Language:English
-
Abstract:
AIMTo evaluate the occurrence and prevalence of microdeletions in the gamma chromosome of patients with azoospermia.
METHODSDNA from 29 men with idiopathic azoospermia was screened by polymerase chain reaction (PCR) analysis with a set of gamma chromosome specific sequence-tagged sites (STSs) to determine microdeletions in the gamma chromosome.
RESULTSDeletions in the DAZ (deleted in azoospermia) loci sgamma254 and sgamma255 were found in three patients with idiopathic azoospermia, resulting in an estimated frequency of deletions of 10.7% in idiopathic azoospermia men.
CONCLUSIONWe conclude that PCR analysis is useful for the diagnosis of microdeletions in the Y chromosome, which is important when deciding the suitability of a patient for assisted reproductive technology such as testicular sperm extracion-intracytoplasmic sperm injection (TESE-ICSI).
