PCR analysis of Yq microdeletions in infertile males, a study from South India.
- Author:
S Ramesh BABU
1
;
M SWARNA
;
P PADMAVATHI
;
P P REDDY
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Chromosome Deletion; Chromosome Mapping; Chromosomes, Human, Y; DNA Primers; Female; Gene Frequency; Genetic Loci; Humans; India; Infertility, Male; genetics; Male; Oligospermia; genetics; Polymerase Chain Reaction; methods; Reference Values; Seminal Plasma Proteins; genetics
- From: Asian Journal of Andrology 2002;4(4):265-268
- CountryChina
- Language:English
-
Abstract:
AIMTo estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India.
METHODSPolymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc.
RESULTSOf the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome.
CONCLUSIONThe frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.
