Expression level of Th22 cells and its cytokines in patients with acute lymphoblastic leukemia and its significance.
10.7534/j.issn.1009-2137.2013.04.009
- Author:
Rong-Hua CHENG
1
Author Information
1. Department of Hematology, Zhengzhou People's Hospital, Zhengzhou 450003, Henan Province, China (E-mail:chenronghua4you@qq.com).
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Case-Control Studies;
Female;
Humans;
Interleukin-6;
blood;
Interleukins;
blood;
Middle Aged;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
metabolism;
RNA, Messenger;
genetics;
Remission Induction;
T-Lymphocytes, Helper-Inducer;
metabolism;
Transforming Growth Factor beta;
blood;
Tumor Necrosis Factor-alpha;
blood;
Young Adult
- From:
Journal of Experimental Hematology
2013;21(4):857-860
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to analyze the expression level of Th22 cells and their cytokines in patients with acute lymphoblastic leukemia (ALL) and evaluate its significance. Forty-eight patients with ALL were selected. According to the treatment, all patients were divided into the newly diagnosed group (n = 26) and complete remission (CR) group (n = 22). The proportion of Th22 cells in peripheral blood was detected by flow cytometry (FCM). The expression levels of cytokines IL-22, IL-6, TNF-α and TGF-β in peripheral blood were measured by ELISA. The expression level of IL-22 mRNA in peripheral blood mononuclear cells was examined by semi-quantitative-reverse transcription PCR (RT-PCR). Meanwhile, 30 healthy individuals were selected as a control group. The parameters of the 3 groups were compared. The results showed that the percentage of Th22 cells and the expression levels of IL-22, IL-6, TNF-α and IL-22 mRNA in newly diagnosed group and the CR group were significantly lower than that in control group, the expression level of TGF-β in above mentioned two group was obviously higher than that in control group (P < 0.05). The percentage of Th22 cells and the expression levels of IL-22, IL-6, TNF-α and IL-22 mRNA in newly diagnosed group were evidently lower than that in CR group (P < 0.05), but the expression level of TGF-β in newly diagnosed group obviously higher than that in CR group. The expression level of IL-22 in newly diagnosed group was positively related with expression level of IL-6 and TNF-α, but it was negatively related with expression level of TGF-β. It is concluded that the decreasing of Th22 cells and down-regulation of IL-22 expression level may be related with pathogenesis of ALL, the decreasing of Th22 cells is risk factor for ALL.
