Effect of curcumin combined with ATRA on differentiation of ATRA-resistant acute promyelocytic leukemia cells.
10.7534/j.issn.1009-2137.2013.04.016
- Author:
Tang-Yong CHEN
1
;
Fen XU
;
Yun-Yuan KONG
;
Fang WEN
;
Fu-Yuan XIE
;
La-Gen WAN
;
Zhang-Lin ZHANG
Author Information
1. Clinical Laboratory, the First Affilianted Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Curcumin;
pharmacology;
Drug Resistance, Neoplasm;
drug effects;
Humans;
Leukemia, Promyelocytic, Acute;
pathology;
Phosphorylation;
Proto-Oncogene Proteins c-akt;
metabolism;
Signal Transduction;
Tretinoin;
pharmacology
- From:
Journal of Experimental Hematology
2013;21(4):895-898
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the effect of curcumin combined with all-trans retinoid acid (ATRA) on differentiation of ATRA-resistant acute promyelocytic leukemia (APL) cells and its molecular mechanism, the NB4-R1, an ATRA-resistant APL cells, was used as a model, counting of NB4-R1 and cell morphologic observation were performed, the effect of curcumin alone or combined with ATRA on proliferation, differentiation of NB4-R1 cells was detected by flow cytometry (FCM), the change of AKT phosphorylation in cell differentiation was detected by Western blot. The results showed that ATRA had no influence on NB4-R1 cell proliferation, but enhanced the inhibitory effect of curcumin on NB4-R1 cell growth; the curcumin or ATRA alone did not affect NB4-R1 differentiation; curcumin combined with ATRA could obviously induce CD11b expression; the cell morphology showed obvious differentiation characteristics. ATRA could promote phosphorylation of AKT in NB4 cells at short time, but not had effect on phosphorylation of AKT in NB4-R1 cells; the curcumin could enhance the phosphorylation of AKT in NB4-1R cells, the curcumin combined with ATRA could further enhance the phosphorylation of AKT. It is concluded that PI3K/AKT pathway inactivation may be one of the factors of drug resistance in APL and curcumin promotes differentiation of NB4-R1 through activating PI3K/AKT pathway.
