Effect of GATA-2 overexpression on function of mouse fetal liver hematopoietic stem cells.
10.7534/j.issn.1009-2137.2013.04.036
- Author:
Jing WU
1
;
Bo WU
;
Yuan-Liang ZHANG
;
Yin-Yin XIE
;
Qiu-Hua HUANG
Author Information
1. Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
Cells, Cultured;
Female;
GATA2 Transcription Factor;
genetics;
Hematopoietic Stem Cells;
cytology;
Liver;
cytology;
Male;
Mice;
Mice, Inbred C57BL
- From:
Journal of Experimental Hematology
2013;21(4):999-1004
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of GATA-2 over-expression on function of mouse fetal liver hematopoietic stem cells. GATA-2 was introduced into mouse fetal liver cells via retrovirus mediated transduction with GFP as a detecting marker. Flow cytometry, colony-forming assay and cell cycle assay were used to detect the biologic changes of these retrovirus infected mouse fetal liver hematopoietic stem cells. The results showed that GATA-2 over-expression increased the Lin(-)Sca1(+)C-Kit(+) (LSK) population dramatically. Cell cycle of LSK cells didn't show abnormal, while colony forming ability decreased significantly. These data indicated that GATA-2 over-expression inhibited definitive differentiation of mouse fetal liver hematopoietic stem cells. It is concluded that over-expression of GATA-2 can significantly raise the LSK cell proportion in mouse fetal liver and inhibit the differentiation capability, the underlying mechanisms may be related to up-regulation of Hes-1, which may lead to the blocking of cell differentiation at the stem/progenitor cell stage.
