Influence of S-nitrosoglutathione on the membrane glycoprotein of frozen platelets.
10.7534/j.issn.1009-2137.2013.04.045
- Author:
Tao WU
1
,
2
;
Jing-Han LIU
;
Chang-Hong ZHANG
;
Hui LI
;
Wu ZHOU
;
Shu-Ying WANG
Author Information
1. Department of Blood Transfusion, Beijing Military Area General Hospital, Beijing 100700, China
2. Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
drug effects;
Blood Preservation;
methods;
Freezing;
Humans;
P-Selectin;
metabolism;
Platelet Activation;
drug effects;
Platelet Glycoprotein GPIb-IX Complex;
metabolism;
Platelet Membrane Glycoproteins;
metabolism;
S-Nitrosoglutathione;
pharmacology
- From:
Journal of Experimental Hematology
2013;21(4):1046-1053
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to explore the influence of S-nitrosoglutathione (GSNO) on membrane glycoprotein of frozen platelet. The levels of membrane glycoprotein on fresh liquid platelets, frozen platelets and frozen platelets with GSNO were measured by flow cytometry. The results showed that the GSNO obviously decreased platelet aggregation, the PAC-1 change in the three groups was not significant. The changes of CD42b and CD62P in fresh liquid platelet group, frozen platelet group and frozen platelets with GSNO were significant different. The change of membrane glycoprotein in above-mentioned three group was not significant. It is concluded that the GSNO inhibits platelet aggregation, maintains the function of platelets and may be used as a cryoprotectant. When frozen platelets were added with GSNO, the molecular rearrangement, structure change and other mechanism may occur in platelets.
