Anti-human 4-1BBL monoclonal antibody stimulates the nuclear translocation of NF-κB and the co-location of 4-1BBL/CD28 isoform in U937 cells.
10.7534/j.issn.1009-2137.2013.05.007
- Author:
Wei ZHANG
1
;
Hong-Xia QIU
;
Tian TIAN
;
Wei-Feng CHEN
;
Ju-Juan WANG
;
Xiang-Chou YANG
;
Ji XU
;
Li-Min DUAN
Author Information
1. Department of Hematology, Department of Geriatrics Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
4-1BB Ligand;
immunology;
metabolism;
Antibodies, Monoclonal;
pharmacology;
CD28 Antigens;
metabolism;
Coculture Techniques;
Humans;
NF-kappa B;
genetics;
Signal Transduction;
U937 Cells
- From:
Journal of Experimental Hematology
2013;21(5):1121-1125
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the molecular mechanism of 4-1BBL reverse signals in the human acute monocytic leukemia cell line of U937. The U937 cell line was used as target cells, and stimulated by the mouse anti-human 4-1BBL monoclonal antibody 1F1. The nuclear translocation of NF-κB and the co-location of 4-1BBL and CD28i molecules in U937 cells were observed with confocal laser scanning microscopy. The protein and m-RNA expression levels of 4-1BBL and CD28i were detected by flow cytometry and RT-PCR respectively. The results showed that the significant nuclear translocation of NF-κB and co-localization of 4-1BBL and CD28i on membrane of U937 cells appeared after being stimulated by mAb1F1. It is concluded that the 4-1BBL reverse signals transduction mediating the growth of U937 cells relates with the nuclear translocation of NF-κB. CD28i may be involved in intracellular 4-1BBL reverse signaling pathways.
