Anti-apoptotic effect of Astragalus Polysaccharide on myeloid cells.
10.7534/j.issn.1009-2137.2013.05.032
- Author:
Bin XIAO
1
,
2
;
Yue XU
;
Han HE
;
Qian-Li JIANG
;
Su-Yi LI
;
Hui-Ying SHU
;
En-Yu LIANG
;
Zheng-Shan YI
;
Jie-Yu YE
;
Lin-Fang HUANG
;
Chang LIU
;
Fan-Yi MENG
;
Mo YANG
Author Information
1. Institute of Hematology, Jinan University, Guangzhou 510632, Guangdong Province, China
2. Department of Hematology, Nanfang Hospital, Southern Medical Uniersity, Guangzhou 510515, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Astragalus Plant;
Caspase 3;
metabolism;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
pharmacology;
HL-60 Cells;
Hematopoiesis;
drug effects;
Humans;
Polysaccharides;
pharmacology;
Thrombopoietin;
pharmacology
- From:
Journal of Experimental Hematology
2013;21(5):1243-1247
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to assess the effect of Astragalus Polysaccharide (ASPS) on in-vitro hematopoiesis. CFU-GM assays were used to determine the effect of ASPS and thrombopoietin (TPO) on granulocytic-monocyte progenitor cells. The CFU assays were also used to investigate the effect of ASPS on the proliferation of HL-60 cells.HL-60 cells were cultured with serum-free RPMI 1640 medium and treated with or without of different concentrations of ASPS. After 72 h incubation, the number of cells were counted.In addition, the caspase-3 and JC-1 expression was determined by flow cytometry with Annexin V/PI double staining. The results showed that ASPS (100, 200 µg/ml) and TPO (100 ng/ml) significantly promoted CFU-GM formation in vitro. Various concentrations of ASPS and TPO also promoted the colony formation of HL-60 cells, the largest effect of ASPS was observed at a concentration of 100 µg/ml. There were no synergistic effects between TPO and ASPS on cellular proliferation. The results also showed that ASPS significantly protected HL-60 cells from apoptosis in condition of serum-free medium culture, suppressed caspase 3 activation, and reduced the cell apoptosis. It is concluded that ASPS can significantly promote the formation of bone marrow CFU-GM and the proliferation of HL-60 cells, the optimal concentration of ASPS is at 100 µg/ml. In the absence of serum inducing apoptosis, ASPS also significantly reduced the apoptosis of HL-60 cells via suppressing the activation of caspase-3.
