Basic biological characteristics of mesenchymal stem cells derived from bone marrow and human umbilical cord.
10.7534/j.issn.1009-2137.2013.05.033
- Author:
Zhen-Xia HAN
1
;
Qing SHI
;
Da-Kun WANG
;
Dong LI
;
Ming LYU
Author Information
1. Department of Cadre Health, Qilu Hospital of Shandong University, Jinan 250005, Shandong Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Bone Marrow Cells;
cytology;
Cell Differentiation;
Cell Separation;
Cells, Cultured;
Humans;
Mesenchymal Stromal Cells;
cytology;
Middle Aged;
Umbilical Cord;
cytology;
Young Adult
- From:
Journal of Experimental Hematology
2013;21(5):1248-1255
- CountryChina
- Language:Chinese
-
Abstract:
Bone marrow (BM) and umbilical cord (UC) are the major sources of mesenchymal stem cells for therapeutics. This study was aimed to compare the basic biologic characteristics of bone marrow-derived and umbilical cord derived-mesenchymal stem cells (BM-MSC and UC-MSC) and their immunosuppressive capability in vitro. The BM-MSC and UC-MSC were cultured and amplified under same culture condition. The growth kinetics, phenotypic characteristics and immunosuppressive effects of UC-MSC were compared with those of BM-MSC.Gene chip was used to compare the genes differentially expressed between UC-MSC and BM-MSC. The results showed that UC-MSC shared most of the characteristics of BM-MSC, including morphology and immunophenotype. UC-MSC could be ready expanded for 30 passages without visible changes. However, BM-MSC grew slowly, and the mean doubling time increased notably after passage 6. Both UC-MSC and BM-MSC could inhibit phytohemagglutinin-stimulated peripheral blood mononuclear cell proliferation, in which BM-MSC mediated more inhibitory effect. Compared with UC-MSC, BM-MSC expressed more genes associated with immune response. Meanwhile, the categories of up-regulated genes in UC-MSC were concentrated in organ development and growth. It is concluded that the higher proliferation capacity, low human leukocyte antigen-ABC expression and immunosuppression make UC-MSC an excellent alternative to BM-MSC for cell therapy. The differences between BM-MSC and UC-MSC gene expressions can be explained by their ontogeny and different microenvironment in origin tissue. These differences can affect their efficacy in different therapeutic applications.
