The effects of AML1B and AML1/ETO on the transactivation of TSC genes..
- Author:
Zhi-Fang XU
1
;
Lin WANG
;
Yang WANG
;
Min WANG
;
Qing RAO
;
Hai-Yan XING
;
Jian-Xiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Binding Sites; Core Binding Factor Alpha 2 Subunit; genetics; Humans; Oncogene Proteins, Fusion; genetics; Plasmids; Promoter Regions, Genetic; Transcription Factors; genetics; Transcriptional Activation
- From: Chinese Journal of Hematology 2009;30(12):804-807
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of AML1B and AML1/ETO fusion gene on the transcription activity of TSC1 and TSC2 promotor and to explore its role in leukemogenesis.
METHODSThe luciferase reporter plasmids of TSCs gene promoter containing a AML1 binding site were constructed, and cotransfected into CV-1 cells with AML1B or AML1/ETO expression plasmids separately. The transactivity of TSCs gene promoter was assayed by luminometer.
RESULTSAML1B exhibited a transactivity to TSC2 gene promoter in a dosage-dependant manner, but showed no significant transactivity to TSC1's. The transactivity to TSC2 gene promoter showed 8.55 fold increase as companed with control group at 75 ng of pCMV5-AML/B. AML1/ETO showed a significant transactivity to TSC1, but no transactivity to TSC2's. However, AML1/ETO antagonized the effect of AMLlB to TSC2 gene promoter.
CONCLUSIONAML1B and AML1/ETO can regulate the transcription of TSC genes.
