The influence of Ara-C on anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells..
- Author:
Ming YANG
1
;
Dong-Mei FAN
;
Ying-Dai GAO
;
Yuan ZHOU
;
Qing JI
;
Xiao-Feng SHAO
;
Jin-Hong WANG
;
Yuan-Fu XU
;
Dong-Sheng XIONG
;
Chun-Zheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cytarabine; Humans; K562 Cells; Leukemia; immunology; T-Lymphocytes; immunology
- From: Chinese Journal of Hematology 2009;30(12):812-815
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Ara-C in regulating anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.
METHODSThe diabody of anti-CD3/anti-Pgp was purified by E-tag affinity chromatography. K562 and K562/A02 cells were treated with Ara-C. The expressions of B7-1 and B7-2 on K562 and K562/AO2 cells were detected by FACS. The cytotoxicity of T-lymphocytes combined with anti-CD3/anU-Pgp plus Ara-C was analyzed by CytoTox 96 nonradioactive method.
RESULTSThe expressions of B7-1 and B7-2 on K562 and K562/A02 cells treated by Ara-C was significantly higher than those untreated. The effect/target ratio was from 0.39:1 to 25:1, and the killing rate of activated T cells to anti-drug-resistant leukemia cells was from (16.44 +/- 1.20)% to (60.49 +/- 2.90)%. The killing rates were increased gradually, with both the effect/target ratio and the antibody concentration increasing (P < 0.05).
CONCLUSIONAra-C may be an important adjuvant for improving anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.
