Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata.
- Author:
Jian-Ping XUE
1
;
Jing-Ying SHI
Author Information
- Publication Type:Journal Article
- MeSH: Achyranthes; cytology; metabolism; Cell Culture Techniques; methods; Plants, Medicinal; cytology; metabolism; Polysaccharides; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(21):2467-2469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.
METHODThe methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.
RESULT AND CONCLUSIONThe results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).
