Content comparison of diterpenoid pigments in different processed products of Gardenia jasminoides.
- Author:
Cun ZHANG
1
;
Yong-Qing XIAO
;
Li LI
;
Gui-Liu LI
;
Zhen PANG
Author Information
- Publication Type:Journal Article
- MeSH: Carotenoids; chemistry; Chromatography, High Pressure Liquid; Diterpenes; chemistry; Drugs, Chinese Herbal; chemistry; Gardenia; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(21):2470-2473
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the contents of diterpenoid pigments in the different processed products of Gardenia jasminoides.
METHODThe separation of Crocin 1, Crocin 2, Crocin 3, Crocetin were determined simultaneously by HPLC on a kromasil C18 column at 35 degrees C with the m methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrase. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL x min(-1).
RESULTThe obtained linearity of the four components was better over 0.9995 and the average recoveries were 97.77%, 100.05% , 98.40%, 101.02%, respectively.
CONCLUSIONThe method is simple, accurate with good reproducibility. The results showed that the remarkable variation regulations appear among the different processed products.
