Isolation and purification of 10-hydroxycamptothecin and vincoside-lactam from Camptotheca acuminata seed by polyamide.
- Author:
Wei-Guo SHI
1
;
Yuan-Gang ZU
;
Lei YANG
;
Chun-Jian ZHAO
;
Jia-Hui LI
Author Information
1. Engineering Research Center of Plant Medical, Northeast Forestry University, Harbin 150040, China.
- Publication Type:Journal Article
- MeSH:
Camptotheca;
chemistry;
Camptothecin;
analogs & derivatives;
chemistry;
isolation & purification;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Hydrogen-Ion Concentration;
Nylons;
chemistry;
Seeds;
chemistry
- From:
China Journal of Chinese Materia Medica
2008;33(21):2486-2489
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the technological parameters of the isolation and purification of 10-hydroxycamptothecin and vincoside-lactam from Camptotheca acuminata seed by polyamide. The static arid dynamic adsorption characteristics of 10-hydroxycamptothecin and vincoside-lactam on polyamide were studied, and the contents were determined by HPLC. The optimum parameters for adsorption were as follows: the contents of 10-hydroxycamptothecin and vincoside-lactam in the extracts were 0.189 g x L(-1) and 0.334 g x L(-1), respectively, pH 6, flow rate was 1.0 mL x min(-1), processing volume was 3 BV; for desorption: ethanol-water (60:40), flow rate was 1.0 mL x min(-1), 5 BV as an eluent. After treated with polyamide, the contents of 10-hydroxycamptothecin and vincoside-lactam were 17.52% and 32.87%, respectively, the recovery yields were 66.05% and 75.86%, respectively. Results showed that polyamide revealed a good ability to separate 10-hydroxycamptothecin and vincoside-lactam. Therefore, we concluded that results in this study may provide scientific references for the large-scale production of 10-hydroxycamptothecin and vincoside-lactam extracted from C. acuminata seed.
