Analysis of principal composition of ethyl acetate part in Huangqi Danggui decoction by HPLC-ESI-TOF-MS.
- Author:
Li-Wei XU
1
;
Ming-Ying SHANG
;
Jun LI
;
Xiao-Mei LI
;
Li-Qiang MENG
;
Shao-Qing CAI
Author Information
- Publication Type:Journal Article
- MeSH: Acetates; chemistry; Amino Acids; chemistry; Benzofurans; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Flavonoids; chemistry; Glycosides; chemistry; Molecular Weight; Oligosaccharides; chemistry; Saponins; chemistry; Spectrometry, Mass, Electrospray Ionization; methods
- From: China Journal of Chinese Materia Medica 2008;33(21):2508-2512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze and identify the principal composition of ethyl acetate part of Huangqi Danggui decoction by high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS).
METHODThe analysis conditions are as follows: Zorbax SB C18 (4.6 mm x 250 mm, 5 microm) column; mobile phase (A) water mobile phase (B) acetonitrile, gradient elution; UV detection wavelength 254 nm; ESI source and data acquisition in positive and negative mode.
RESULTThe accurate molecular weights of 20 compounds were measured and identified in ethyl acetate part of Huangqi Danggui decoction. Furthermore, the types of them are as below: flavonoids and flavonoid glycosides, saponins, oligosaccharides, amino acids and phthalides.
CONCLUSIONIt is a rapid and accurate method that the compositions of compound prescription of traditional Chinese medicine can be identified in terms of the separation of high performance liquid chromatography, the accurate molecular weights measured by MS and other information, which can clarify the potential effective compounds of Huangqi Danggui decoction.
