ESR study and protection of water extract of Carthamus tinctorius on ox-LDL induced injury in rat cardiac microvascular endothelial cell.
- Author:
Jin-Xia YE
1
;
Ri-Xin LIANG
;
Lan WANG
;
Bin YANG
;
Rong-Shu AN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carthamus tinctorius; chemistry; Drugs, Chinese Herbal; chemistry; pharmacology; Electron Spin Resonance Spectroscopy; methods; Endothelial Cells; drug effects; metabolism; Enzyme Activation; drug effects; Glutathione Peroxidase; metabolism; L-Lactate Dehydrogenase; metabolism; Lipoproteins, LDL; pharmacology; Microvessels; cytology; Nitric Oxide Synthase; metabolism; Rats; Rats, Wistar; Reactive Oxygen Species; metabolism; Superoxide Dismutase; metabolism; Water; chemistry; Xanthine Oxidase; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(21):2513-2517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the antioxidant effects of water extract of Carthamus tinctorius on ox-LDL induced injury in rat cardiac microvascular endothelial cell and detecting oxygen derived free radicals (OFR) to explore the antioxidant mechanisms.
METHODBy using the third generation of rat cardiac microvascular endothelial cells (rCMEC), the protection of water extract of C. tinctorius was investigated after ox-LDL (100 mg x L(-1) induced damage. The supernatant was collected for detecting lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD), glutathione peroxidase (GSH-Px), nitric oxide (NO), nitric oxide synthase (NOS) activity, and cell suspension was collected for detecting reactive oxygen species (ROS) by electron spin resonance (ESR).
RESULTWater extract of C. tinctorius increased the rCMEC survival rate, reduced LDH, MDA and XOD levels, and improved SOD, GSH-Px and NOS activity, while in the cell suspension ROS signal decreased significantly.
CONCLUSIONWater extract of C. tinctorius has antioxidation. The mechanisms are likely related with scavenging of free radicals, enhancing its clearance, enhancing endogenous antioxidant activity.
