Study on genetic relationship of official Rheum by SRAP.
- Author:
Da-Xia CHEN
1
;
Long-Yun LI
;
Guo-Yue ZHONG
;
Song-Yun QIN
;
Chang-Hua WANG
;
Zai-Bo YU
Author Information
- Publication Type:Journal Article
- MeSH: Cluster Analysis; Genetic Variation; genetics; Phylogeny; Polymerase Chain Reaction; Polymorphism, Genetic; genetics; Rheum; classification; genetics
- From: China Journal of Chinese Materia Medica 2008;33(20):2309-2312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the genetic relationship of three species of official Rheum in molecular level.
METHODTwelve samples from three species of official Rheum were employed to be analyzed by the approach of sequence-related amplified polymorphism (SRAP). Systematic relationships were constructed based on the UPGMA method by TREECONW software.
RESULTA total of 272 bands were scored and 199 bands of them were polymorphic, which were up to 73.2% polymorphic ratio. Genetic similarity coefficient was changed from 0.578 4 to 0.941 6. The results indicated that there was abundant genetic diversity among the tested materials. The clustering analysis revealed that the results between SRAP marker and the traditional morphological characteristics was almost the same.
CONCLUSIONSRAP marker is suitable for variety identification and genetic relationship research in official Rheum.
