Optimal extraction of nuciferine and flavone from lotus leaf based on central composite design and response surface methodology.
- Author:
Guo-Hua WANG
1
;
Bao-Xian ZHANG
;
Qi-Xia NIE
;
Hui LI
;
Chen ZANG
Author Information
- Publication Type:Journal Article
- MeSH: Aporphines; chemistry; isolation & purification; Flavones; Flavonoids; chemistry; isolation & purification; Loteae; chemistry; Plant Leaves; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(20):2332-2335
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the process of extracting effective constituents from lotus leaf.
METHODIndependent variables were ethanol concentration reflux time and solvent fold, dependent variables were extraction rates of nuciferine and flavone in lotus leaf, central composite design and response surface methodology were used for optimization of extraction of lotus leaf.
RESULTThe optimum conditions of extraction process were 75% -80% ethanol, 2-3 hours for reflux, 20-25 fold solvent and 2 times for extraction. Bias between observed and predicted of rates of nuciferine and flavone values were 5.53%, -6.02%, respectively.
CONCLUSIONThe values observed and predicted were close to each other, which proved that the optimization of of extraction of lotus leaf by central composite design and response surface methodology was reasonable and successful.
