Study on fingerprints of Citrus aurantium from different places by capillary electrophoresis.
- Author:
Guang-Ming LUO
1
;
Xiao-Hui TAN
;
Li-Fang XU
;
Ya-Qin YANG
;
Shi-Lin YANG
Author Information
- Publication Type:Journal Article
- MeSH: Borates; chemistry; Citrus; chemistry; Electrolytes; chemistry; Electrophoresis, Capillary; methods; Hydrogen-Ion Concentration; Temperature
- From: China Journal of Chinese Materia Medica 2008;33(20):2362-2364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.
METHODThe background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.
RESULTThere were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.
CONCLUSIONThe CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.
