Determination of paeoniflorin in rat plasma by HPLC-MS/MS and its pharmacokinetics.
- Author:
Jie WU
1
;
Nan YAO
;
Da-Wei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzoates; administration & dosage; blood; pharmacokinetics; Bridged-Ring Compounds; administration & dosage; blood; pharmacokinetics; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; metabolism; pharmacokinetics; Glucosides; administration & dosage; blood; pharmacokinetics; Humans; Male; Monoterpenes; Paeonia; chemistry; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry; methods
- From: China Journal of Chinese Materia Medica 2008;33(20):2369-2373
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEAn HPLC-MS/MS assay was established to determine paeoniflorin in rat plasma and be used to investigate the effect of pharmacokinetics of paeoniflorin when co-administrated Radix Angelicae Sinensis and Radix Paeoniae Rubra.
METHODHPLC-MS/MS was performed in the multiple reaction monitoring (MRM) mode using target ions at m/z 450 --> m/z 327 for paeoniflorin and m/z 388 --> m/z 225 for jasminoidin. A single dose of Radix Paeoniae Rubra alone and with Radix Angelicae Sinensis was given to rats by ig, the dosage of paeoniflorin was 294.78 mg x kg(-1). Concentrations of paeoniflorin in rat plasma were determined by HPLC-MS/MS assay and main pharmacokinetic parameters were estimated.
RESULTThe mainly pharmacokinetic parameters of paeoniflorin when administrated Radix Paeoniae Rubra only were as follows: C(max) (1.55 +/- 0.53) mg x L(-1), T(max) (0.9 +/- 0.3) h, t 1/2 (1.51 +/- 0.63) h, MRT (3.08 +/- 0.74) h, AUC(0 --> tau) (4.68 +/- 0.85) mg x h(-1) x L(-1). When the two drugs administrated together, the corresponding parameters were (0.93 +/- 0.42) mg x L(-1), (1.5 +/- 0.8) h, (3.08 +/- 1.79) h, (5.19 +/- 1.95) h, (3.36 +/- 0.56) mg x h(-1) x L(-1), respectively. The parameters of C(max), AUC(0 --> tau) and MRT were significantly changed (P < 0.05).
CONCLUSIONRadix Angelicae Sinensis can significantly affect the pharmacokinetics of paeoniflorin, the principal component of Radix Paeoniae Rubra.
