Effect of oxymatrine on NF-kappaB and other cell factors in rats lung tissue with septic shock.
- Author:
Ming-Hao ZHANG
1
;
Gui-Zhong LI
;
Hua XU
;
Juan ZHANG
;
Jun CAO
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Animals; Anti-Arrhythmia Agents; pharmacology; Gene Expression Regulation; drug effects; I-kappa B Proteins; metabolism; Immunohistochemistry; Interleukin-6; metabolism; Lung; drug effects; metabolism; pathology; Male; NF-kappa B; metabolism; Quinolizines; pharmacology; Radioimmunoassay; Random Allocation; Rats; Rats, Sprague-Dawley; Shock, Septic; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(20):2390-2394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of oxymatrine (OMT) on NF-kappaB and other cell factors in rat lung tissue with septic shock.
METHODFifty-six male SD rats were randomly divided into 7 groups: sham operation group, OMT control group, model (CLP) group, CLP + OMT high, middle, low-dose group, positive control group. Changes in NF-kappaB (p65) and IkB-alpha activity in the pulmonary tissue were determined by immunohistochemical method, tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels in pulmonary tissue were determined by radioimmunoassay.
RESULTOMT could decrease significantly the NF-kappaB (p65) and IkB-alpha activity in the pulmonary tissue (P < 0.05), TNF-alpha and IL-6 levels in pulmonary tissue homogenate decreased markedly (P < 0.05). OMT could elevate the content of PaO2, SaO2, decrease the content of PaCO2, HCO3- and decrease the ratio between wet weight of the lung and dry weight of the lung and the PWI.
CONCLUSIONOMT can inhibit NF-kappaB-inducing kinase (NIK), NF-kappaB activity and reduce the expression of proinflammatory factor (TNF-alpha, IL-6) and antagonize the lung injury in a rat model of septic shock.
