Content determination of epigoitrin in Radix Isatidis and its preparation by RP-HPLC.
- Author:
Yi-Qiang AN
1
;
Xiao-Bin JIA
;
Hai-Jian YUAN
;
E SUN
;
Zhen-Zhen XU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Isatis; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(18):2074-2076
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for the content determination of epigoitrin in Radix Isatidis and its preparation, and to provide valuable data for quality control of Radix Isatidis and its preparation.
METHODThe samples were separated on a ZORBAX SB-C18 (4. 6 mm x 150 mm, 5 microm) column with the mobile phase of acetonitrile-water-phosphoric acid-triethylamine (8.50 : 90.72 : 0.73 : 0.05) in the flow rate of 0.7 mL x min(-1). The detection wavelength was set at 245 nm. Column temperature was 30 degrees C.
RESULTThe linear range of epigoitrin was 0.0204-0.3060 microg (r = 0.9998), and the average recovery was 98.99% with the RSD was 1.31% (n = 9).
CONCLUSIONThe method for quantitation of epigoitrin in Radix Isatidis and its preparation was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis and its preparation.
