Studies on constituents of rootsanel leaves from Desmodium blandum and their cytotoxic activity against growth of several tumor cells.
- Author:
Ning GAN
1
;
Xin YANG
;
Tian-Hua LI
;
Ping HE
Author Information
- Publication Type:Journal Article
- MeSH: Alkenes; chemistry; pharmacology; Benzene Derivatives; chemistry; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; chemistry; toxicity; Fabaceae; chemistry; Humans; Magnetic Resonance Spectroscopy; Molecular Structure; N,N-Dimethyltryptamine; chemistry; Plant Leaves; chemistry; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Infrared
- From: China Journal of Chinese Materia Medica 2008;33(18):2077-2080
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the chemical constituents of Desmodium blandum and their cytotoxic activity against the growth of several tumor cells.
METHODVarious chromatographic techniques including silica gel, Sephadex LH-20 column chromatography were employed for the isolation and purification of the constituents. The structures of compounds were elucidated by spectral analyses (IR, UV, NMR, MS). Their cytotoxic activity was then studied.
RESULTEight compounds were isolated from the stems of D. blandum and identified as N, N-dimethyltryptamine (1), 5-methoxy-N, N-dimethyltryptamine (2), citrusinol (3), yukovanol (4), (Z)-1-(4-hydroxy-2, 3-dimethoxyphenyl)-3-(4-hydroxyphenyl) propene (5), (Z)-1-(3-hydroxy-2, 4-dimethoxy-phenyl)-3-(4-hydroxy-3-methoxy-phenyl) propene (6), methylprotocatechuate (7), katuranin (8).
CONCLUSIONAmong these compounds, compound 6 was isolated from D. blandum for the first time. In the MTT test, compounds 2 and 6 exhibit cytotoxic activities against the KB cell, and compounds 3 and 6 exhibit the same activities against the HepG2 cell.
