Study on HPLC fingerprint of Prunella vulgaris.
- Author:
Wei-Guang SUN
1
;
Xue-Hong KE
;
Yuan LI
;
Ru-Feng HUA
;
Jin-Fu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Prunella; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(18):2090-2093
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.
METHODA sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.
RESULTThe similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.
CONCLUSIONThis method is available for quality evaluation and control the quality of P. vulgaris.
