OBJECTIVETo develop an HPLC method for determination of two major secoiridoid glucosides (nuezhenoside and G13) from Fructus Ligustri Lucidi. and determinae nuezhenoside and G13 in samples from different acquisition times and different places.

METHODThe sample was separated on a YMG-C18 (4.6 mm x 250 mm, 10 microm) column. The mobile phase was acetonitrile-water, gradient elution (CH3CN: 0 min, 20%; 15 min, 25%) with the flow rate of 1 mL x min(-1), the detection wavelength was 240 nm, and the column temperature was set at 25 degrees C.

RESULTIn HPLC analysis ,the linear ranges of nuezhenoside and G13 were 0.341-34.1 microg and 0.331-33.1 microg, respectively; their average recoveries were 97.5% (RSD 1.35%) and 98.1% (RSD 1.82%), respectively. The results of content determination (nuezhenoside and G13) of samples from different places varied greatly. This may be caused by different species sources and different preparation methods, etc. The experiment led up to the fact that from the beginning to the middle of November the content of nuezhenoside and G13 reached the maximum.

CONCLUSIONThe established method can be used to determine two major secoiridoid glucosides (nuezhenoside and G13) in Fructus Ligustri Lucidi simultaneously. Meanwhile it can be used for the quality control of Fructus Ligustri Lucidi.