RP-hPLC determination of flavonoids in several flowers.
- Author:
Ying-Feng NIU
1
;
Yun SHAO
;
Xiao-Hui ZHAO
;
Huai-Xiu WEN
;
Yan-Duo TAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Flavonoids; chemistry; Flowers; chemistry; Iris Plant; chemistry; Kaempferols; chemistry; Prunus; chemistry; Quercetin; chemistry; Rosa; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(18):2102-2104
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develvp a RP-HPLC method for the determination of flavonoids in fifteen kinds of flowers such as Iris lacteal pall, prunus persica and rosa chinensis.
METHODThe contents of quercetin, kaempferol and isorhamntin in fifteen kinds of flowers were extracted with methanol. The analysis was performed on a Kromasil C18 column (4.6 mm x250 mm, 5 microm) with methanol-0.1% phosphoric acid (50:50) as mobile phase.
RESULTThe quercetin, kaempferol and isorhamntin were separated well, and the result shows that the content of quercetin in the Iris lactea Pall was the highest (1.536%), the contene of kaempferol in Persica persice was the highest (0.572%), and the content of isorhamntin in chrysamthemum morifolium was up to 0.290%.
CONCLUSIONThe contents of flavonoids in these flowers were by determined RP-HPLC for the first time and the method can be used for quantitative determination of flavonoids in the flowers.
