Morroniside inhibits H2O2-induced apoptosis in cultured nerve cells.
- Author:
Hou-Xi AI
1
;
Wen WANG
;
Fang-Lin SUN
;
Wen-Ting HUANG
;
Yi AN
;
Lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Blotting, Western; Caspase 3; metabolism; Caspase 9; metabolism; Cell Line, Tumor; Enzyme Activation; drug effects; Glycosides; pharmacology; Humans; Hydrogen Peroxide; pharmacology; Mice; Neurons; cytology; drug effects; metabolism; Oxidants; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Superoxide Dismutase; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(18):2109-2112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of morroniside on H2O2-induced apoptosis in nerve cells.
METHODHuman neuroblastoma cell line SH-SY5Y cells were pre-incubaed with morroniside (1, 10, and 100 micromol x L(-1)) for 24 h prior to exposure to H2O2 (500 micromol x L(-1)) for 18 h. The activity of reactive SOD was measured by a biochemical assay. The expression of caspase-3, caspase-9, Bcl-2 and Bax was determined by Wastern blotting method.
RESULTPretreatment of the cells with morroniside (10 and 100 micromol x L(-1)) increasd SOD activity by 14% (P<0.01) and 11% (P<0.05) in comparison with cells exposed only to H2O2. Morroniside (1, 10, 100 micromol x L(-1)) lowered caspase-3 level by 31% (P<0.01), 103% (P<0.001) and 95% (P<0.001), decreased caspase-9 content by 71% (P<0.001), 132% (P<0.001) and 37% (P<0.05), and increasd Bcl-1 level by 88% (P<0.01), 121% (P<0.001) and 60% (P<0.01) respectively but no significant change occurred in Bax level in comparison with cells exposed only to H2O2.
CONCLUSIONMorroniside has neuroprotection effect against H2O2-induced oxidation injury in nerve cell.
