Identification of Gynostemma from their adulterant Cayratia japonica by PCR-RFLP.
- Author:
Chong WANG
1
;
Yuan-Yuan ZHANG
;
Juan YANG
;
Ling-Yan JIANG
;
Miao-Miao LI
;
Gui-Fang ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Chloroplast; genetics; DNA, Plant; genetics; Gynostemma; classification; genetics; Polymerase Chain Reaction; methods; Polymorphism, Restriction Fragment Length; genetics; Vitaceae; classification; genetics
- From: China Journal of Chinese Materia Medica 2008;33(19):2167-2170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a convenient and effective method for the identification of Gynostemma and Cayratia japonica.
METHODEight species, including Gynostemm pentaphyllum, G. pentagynum, G. cardiospermum, G. longipe, G. yixingense, G. laxiflorum, G. guangxiense and C. japonica were investigated through PCR - RFLP of six chloroplast DNA fragments. The six gene fragments were digested by six restriction endonuclease respectively, including Taq I, Hpa II, EcoR I, Rsa I, Hha I, Hind III.
RESULTSeven species of Gynostemma and their adulterant could be identified by trnK1f-trnK2r and Rsa.
CONCLUSIONPCR - RFLP provides a quick, reliable molecular marker technique for identification of Cynostemma and their adulterant Cayratia japonica.
