Rapid determination of contents of hirudin's hydrolysates in processed leech by dot blotting.
- Author:
Hou-Wei WANG
1
;
Jing-Zhen TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; chemistry; Hirudins; immunology; metabolism; Immunoblotting; methods; Leeches; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(19):2193-2195
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the contents of hirudin's hydrolysates in processed leeches, set up a new evaluating method of dot blotting to evaluate the qualities of those processed Chinese medicines as leeches.
METHODContents of hirudin's hydrolysates in processed leeches were determined by dot blotting with rat antibody of anti-hirudin as the first antibody. Blotting signal was analyzed by software of Quantity One.
RESULTContents of hirudin's hydrolysates in four batches of processed leeches were 296.51, 165.47, 95.58, and 298.05 microg g(-1), respectively.
CONCLUSIONDifference among four batches of processed leeches was significant in the content of hirudin's hydrolysates. Dot blotting, as a convenient and accurate method can be broadly used for evaluating processed products of Chinese crude drugs similar to leeches.
