Sodium dodecyle sulphate polyacrylamide gel electrophoresis of whole salivary proteins from patients with adult periodontitis.
- Author:
Xiaolin TANG
1
;
Yaping PAN
;
Zhaoyuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Electrophoresis, Polyacrylamide Gel; methods; Female; Humans; Male; Middle Aged; Periodontitis; metabolism; Saliva; chemistry; Salivary Proteins and Peptides; analysis; Sodium Dodecyl Sulfate
- From: West China Journal of Stomatology 2003;21(2):98-100
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was intended to determine the salivary protein factors related to adult periodonititis.
METHODSTwenty-five patients with adult periodontitis (AP group) and twenty-five normal controls (NC group, 25) were adopted in the study. Salivary protein composition in unstimulated whole saliva samples obtained before and after basic treatment was analyzed by SDS-PAGE.
RESULTSThe volume of proteins with 66 kD, 18 kD, 15 kD, 13 kD in AP group before treatment were all remarkably higher than those in NC group. In addition, 51 kD, 34 kD, 19 kD, 17 kD protein bands were more frequently observed in AP group by comparison with NC group. However, after initial therapy, all the variables mentioned above decreased remarkably.
CONCLUSIONThe results demonstrated that protein bands with 18 kD, 15 kD, 13 kD might be closely related to the occurrence and recovery of periodontitis, and serum-derived proteins in saliva increased in patients with adult periodontitis.
