Immunohistochemical research of integrin alpha 5 expression during mandibular fracture healing.
- Author:
Shaohua LIU
1
;
Shengwei LI
;
Gang CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Fracture Healing; Immunohistochemistry; In Situ Hybridization; Integrin beta1; biosynthesis; genetics; Male; Mandibular Fractures; metabolism; Osteoblasts; physiology; RNA, Messenger; biosynthesis; genetics; Rabbits
- From: West China Journal of Stomatology 2003;21(2):101-103
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression change of integrin alpha 5 during mandibular fracture healing.
METHODSUsing rabbit mandibular fracture model, the fractured bone tissues were obtained and paraffin slices were made on the days of 1, 3, 5, 7, 14, 30, 60 and 90 after surgery, respectively. Non-fractured mandibles were used as normal control. LsAB immunohistochemical method was used to detect the expression of integrin alpha 5 in bone tissue, especially on osteoblasts and osteoclasts.
RESULTSIntegrin alpha 5 was widely expressed in fractured bone and surrounding soft tissues. The expression of integrin alpha 5 increased 7 days after fracture, peaked in 14 to 30 days and returned to nearly normal in 60-90 days.
CONCLUSIONDuring mandibular fracture healing, the expression of integrin alpha 5 in bone tissue increased clearly. It is estimated that integrin alpha 5 players an important role in fracture healing.
