The effect of anti-ICAM-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lymphocytes from oral squamous cell carcinoma.
- Author:
Hongyu YANG
1
;
Juan LUO
;
Jinrong LI
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Monoclonal; pharmacology; Carcinoma, Squamous Cell; immunology; pathology; Cells, Cultured; Cytotoxicity, Immunologic; Female; Humans; Intercellular Adhesion Molecule-1; biosynthesis; genetics; Lymphocyte Function-Associated Antigen-1; biosynthesis; genetics; Lymphocytes, Tumor-Infiltrating; immunology; pathology; Male; Mouth Neoplasms; immunology; pathology
- From: West China Journal of Stomatology 2003;21(2):107-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of anti-ICAm-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lymphocytes from oral squamous cell carcinoma.
METHODSTIL were isolated from fresh tumor tissues in 15 patients with oral squamous cell carcinoma and cultured in vitro. The expression of ICAM-1/LAF-1 in fresh and activated TIL was examined by FACS. The cytotoxicity of TIL against auto tumor cells and the level of TNF-alpha produced by TIL were compared before and after interrupted with anti-ICAM-1/LAF-1 McAb.
RESULTSThe expression of ICAM-1 and LAF-1 in the activated TIL significantly increased comparing with fresh TIL (P < 0.05). Interrupted by anti-ICAM-1 McAb, the cytotoxicity of TIL against auto tumor cell was not significantly changed. Interrupted by anti-ICAM-1 and anti-LAF-1 McAb, the level of TNF-alpha produced by TIL showed no differences comparing with the control (P < 0.05), but the cytotoxicity of TIL against auto tumor cells significantly decreased (P < 0.05).
CONCLUSIONThe effect of anti-LAF-1 McAb may result from steric interference of antigen-nonspecific adhesion process.
