Construction of the eukaryotic expression vector PsecTaq2A-AMG for human amelogenin.
- Author:
Ailing YANG
1
;
Chenrong XU
;
Jincai ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amelogenin; Clone Cells; metabolism; Cloning, Molecular; DNA, Recombinant; biosynthesis; genetics; Dental Enamel Proteins; biosynthesis; genetics; Escherichia coli; genetics; metabolism; Eukaryotic Cells; metabolism; Genetic Vectors; Humans; Plasmids; genetics; Recombinant Proteins; biosynthesis; genetics
- From: West China Journal of Stomatology 2003;21(2):133-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study was to construct a eukaryotic expression vector for human amelogenin (AMG).
METHODSPCR was performed to amplify the AMG encoding region. Amplified fragments for human AMG were recovered and inserted into eukaryotic expression vectors PsecTaq2A. The recombinant plasmid PsecTaq2A-AMG was constructed and their positive clones were identified.
RESULTS1. Amplified products were checked by electrophoresis and the results were satisfactory. 2. The recombinant plasmid PsecTaq2A-AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank.
CONCLUSIONThe recombinant plasmid PsecTaq2A-AMG was successfully constructed with properly inserted DNA sequence encoding mature amelogenin.
