The effect of heat shock on the expression of urokinase-type plasminogen activator in human tongue squamous cell carcinoma cell line (Tca8113).

Author: Xinhua LIANG ¹ ; Guizhou XIAO ; Zuyi MAO
Author Information

1. Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Publication Type:Journal Article
MeSH: Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Flow Cytometry; Hot Temperature; Humans; Hyperthermia, Induced; Immunohistochemistry; Lymphatic Metastasis; Neoplasm Invasiveness; Tongue Neoplasms; metabolism; pathology; Urokinase-Type Plasminogen Activator; analysis; biosynthesis
From: West China Journal of Stomatology 2003;21(2):150-152
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThe aim of this study was to observe the expression of urokinase-type plasminogen activator (UPA) in human tongue squamous cell carcinoma cell line (Tca8113) after heat shock with different dosage.
METHODSThe expression of UPA protein of Tca8113 after different heating temperatures(37 degrees C, 40 degrees C, 43 degrees C and 45 degrees C) treatment was examined by immunohistochemical technique (IH) and flow cytometry (FCM).
RESULTSCompared with 37 degrees C group, the UPA protein expression in 40 degrees C and 43 degrees C groups decreased significantly (P < 0.05); however, the UPA protein expression in 45 degrees C group decreased but no statistical difference was found.
CONCLUSIONHyperthermia could inhibit invasion and metastasis of oral squamous cell carcinoma and could be considered as a safe method in curing the tumor.