The effect of gene transfection of vascular endothelial growth factor-C on the metastasis ability of Tca8113.
- Author:
Chengge HUA
1
;
Jian PAN
;
Yuming WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Squamous Cell; genetics; pathology; Lymphatic Metastasis; prevention & control; Male; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Random Allocation; Tongue Neoplasms; genetics; pathology; Transfection; Vascular Endothelial Growth Factor C; genetics
- From: West China Journal of Stomatology 2003;21(3):171-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role that vascular endothelial growth factor-C plays in the lymph node metastasis of the squamous cell carcinoma (SCC) of tongue.
METHODSAn established VEGF-C transfected Tca8113 cell strain (VcTca) was heterotransplanted into BALB/C nude mice subcutaneously. The expression of VEGF-C and VEGFR-3 was detected. The property of tumorigenesis and metastasis of the transfected cell was investigated.
RESULTSJugular lymphatic metastases developed in 4/6 mice of VcTca group in 6 weeks after transplantation, while no lymph node metastasis was found in mice with tumors of non-transfected Tca8113 cell (Tca) and mock transfected cell(pDTca) at the same time. There was no difference in tumor weight among VcTca, pDTca and Tca (P > 0.05).
CONCLUSIONIt is suggested that VEGF-C could promote lymph node metastatic ability of Tca8113 cell, and could be utilized as a target of new regimen in prevention and treatment for lymphatic metastasis of oral carcinomas.
