The effects of nm23-H1 on metastases ability and chemo-sensitivity in Tca8113 cell lines.
- Author:
Shaowei CHEN
1
;
Hongzhang HUANG
;
Jian PAN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Carcinoma, Squamous Cell; genetics; pathology; Cisplatin; pharmacology; Drug Resistance, Neoplasm; Gene Expression Regulation, Neoplastic; Humans; NM23 Nucleoside Diphosphate Kinases; Neoplasm Metastasis; Nucleoside-Diphosphate Kinase; Proteins; genetics; Tongue Neoplasms; genetics; pathology; Transfection; Tumor Cells, Cultured
- From: West China Journal of Stomatology 2003;21(3):180-183
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study was to establish a stable, high-efficient and low-toxic way of transfecting nm23-H1 into Tca8113 line cells, and then to find out whether nm23-H1 could affect the invasion and metastases ability of Tca8113 line cells.
METHODSnm23-H1 was transfected into Tca8113 line cells with Lipofect. The different expressions of nm23-H1 between transfected and non-transfected line cells were detected by the method of immunohistochemistry. The difference of the invasion and metastases ability between transfected and non-transfected line cells was detected by transwell-room and wash techniques. The change of chemo-sensitivity was evaluated by MTT.
RESULTSUsing pCMV-NEO-BAM system to keep stable expression of nm23-H1, the significant difference of NDPKA expression between transfected and non-transfected Tca8113 line cells was discovered; The metastases ability of transfected Tca8113 line cells decreased significantly; The chemo-sensitivity of transfected Tca8113 line cells to CDDP increased significantly.
CONCLUSIONnm23-H1 can inhibit the metastases of Tca8113 line cells and increase the chemo-sensitivity to CDDP significantly.
