The effect of telomerase inhibitors on oral squamous carcinoma cells.
- Author:
Zhiqiang YANG
1
;
Wen CHEN
;
Qiao ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Carcinoma, Squamous Cell; pathology; Cell Division; drug effects; Enzyme Inhibitors; pharmacology; Humans; Mouth Neoplasms; pathology; Oligodeoxyribonucleotides; pharmacology; Telomerase; antagonists & inhibitors; metabolism; Thionucleotides; Tumor Cells, Cultured; Zidovudine; pharmacology
- From: West China Journal of Stomatology 2003;21(3):231-234
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the potential anti-tumor effect of telomerase inhibitors.
METHODSHuman oral squamous carcinoma cell line KB was selected as target cell. The effects of 3'-azido-3'-deoxythymidine (AZT) and human antisense phosphorothioate (AS-ONS) for telomerase template on KB cell line were investigated. The cytotoxic effect of AZT and AS-ONS on tumor cells was quantified using MTT colorimetric assay. Assay of 3H-TdR incorporation was undertaken to measure the cell proliferation. The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi-quantitative analysis. The Flow Cytometry was used to detect apoptosis and measure cell cycle.
RESULTSBoth AZT and AS-ONS inhibited the growth of KB cell line in a certain range of concentrations, and meanwhile the telomerase activity was reduced after treatment. In addition, both AZT and AS-ONS can induce apoptosis and arrest G1 phase of cell cycle.
CONCLUSIONThe results obtained above indicated that AZT and AS-ONS could be potentially used as an anti-oral carcinoma agent or an auxiliary treatment for cancer. Those inhibitory effects might be partially due to the induction of apoptosis and the prolongation of cell cycle.
