Cloning and sequencing of human vascular endothelial growth factor-C encoded cDNA.
- Author:
Jian PAN
1
;
Chengge HUA
;
Yuming WEN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; Cloning, Molecular; DNA, Complementary; genetics; Humans; Sequence Analysis, DNA; Tongue Neoplasms; genetics; Vascular Endothelial Growth Factor C; genetics
- From: West China Journal of Stomatology 2003;21(4):318-320
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA functional VEGF-C cDNA was cloned from a patient with squamous cell carcinoma (SCC) of tongue in order to study the important role of vascular endothelial growth factor (VEGF)-C in lymphatic dissemination of malignancies in gene level.
METHODSRT-PCR was employed to clone the human VECF-C encoded cDNA from a surgical specimen of a lingual SCC patient. Then it was subcloned into plasmid vector pCRII and sequenced.
RESULTSA 1.1 kb human VEGF-C cDNA fragment was amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99.6% similarity with the reported human VEGF-C cDNA (representing the 559-1,611 bp according the sequence of Genbank Entry X94216).
CONCLUSIONAn encoded fragment VEGF-C cDNA was successfully cloned from a lingual SCC and provided a necessary material for further study.
