Expression of matrix metalloproteinase-3 and tissue inhibitor metalloproteinases-1 in regenerated rabbit bone after mandibular osteodistraction.
- Author:
Shujuan ZOU
1
;
Zhiguo WANG
;
Jing HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Regeneration; Bone Remodeling; Female; Male; Mandible; cytology; metabolism; surgery; Matrix Metalloproteinase 3; biosynthesis; Osteoblasts; metabolism; Osteogenesis, Distraction; Rabbits; Tissue Inhibitor of Metalloproteinase-1; biosynthesis
- From: West China Journal of Stomatology 2003;21(5):342-363
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression patterns of matrix metalloproteinase-3 and its inhibitor, tissue inhibitor metalloproteinases (TIMP)-1, in remodeling phase of mandibular distraction osteogenesis.
METHODSBilateral mandibular osteotomies were performed in 8 mature rabbits. The mandibles were lengthened 6 mm using a custom-made distractor with a rate of 1 mm/d. All animals were killed in 4 weeks after completion of distraction. The distracted calluses were harvested and processed for histology and immunohistochemistry of MMP-3 and TIMP-1.
RESULTSElevated expression of both MMP-3 and TIMP-1 was found in the distracted calluses resulting from mandibular lengthening. Positive staining for MMP-3 was seen in the osteoblasts and osteocytes, and TIMP-1 was mainly localized in osteoblasts.
CONCLUSIONMMP-3 and TIMP-1 appear to play important roles in the remodeling of new bone created by mandibular distraction osteogenesis.
