DNA sequence analysis for the promoter of pyruvate oxidase gene from Streptococcus oralis.
- Author:
Jincai ZHANG
1
;
Rong ZHANG
;
Yunhui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Cloning, Molecular; Escherichia coli; genetics; Genes, Bacterial; Humans; Hydrogen Peroxide; metabolism; Molecular Sequence Data; Plasmids; genetics; Promoter Regions, Genetic; genetics; Pyruvate Oxidase; genetics; Sequence Analysis, DNA; Streptococcus oralis; enzymology; genetics
- From: West China Journal of Stomatology 2003;21(5):350-352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo elucidate the molecular structure of pyruvate oxidase gene promoter.
METHODSThe 1.30 kb fragment with promoter activity, amplified from upstream of Streptococcus oralis pyruvate oxidase gene (Sopox), was cloned into vector PBK-CMV. The positive transformed E. coli JM109 clone was selected, the recombinant plasmid was further identified with restriction mapping analysis. The positive recombinant plasmid was studied with sequence analysis.
RESULTSAfter digesting the recombinant plasmid with Hind III, 1% agarose electrophoresis showed 1.30 kb fragment, which was consistent with predicted size. Sequence analysis revealed 1,350 bp.
CONCLUSIONThe Sopox promoter region is sequenced. Further characterization of the Sopox promoter region will elucidate the molecular mechanism of H2O2 production of streptococcus oralis.
