The study of peptide vaccine HDS from Streptococcus mutans glucosyltransferase.
- Author:
Yun DING
1
;
Junqi LING
;
Han CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dental Caries; prevention & control; Glucosyltransferases; genetics; immunology; Male; Peptides; immunology; Rats; Rats, Sprague-Dawley; Streptococcal Vaccines; immunology; Streptococcus mutans; enzymology; genetics; immunology
- From: West China Journal of Stomatology 2003;21(5):353-355
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the antigenicity of the peptide vaccine HDS from Streptococcus mutans glucosyltransferase and its ability to induce protective immune responses in an experimental rat model of dental caries.
METHODSArtificial antigen HDS-KLH, peptide HDS, glycosyltransferase were injected to immunize rats. Measurement of the specific anti-HDS, GTF IgG or IgA concentration in saliva and serum were undertaken by ELISA among the experimental groups. Gnotobiotic rat model was developed when challenged S. mutans and a caries promoting diet. The jaws of the rats were selected and dyed. The Keyes caries score for each jaw were counted.
RESULTSThe level of serum and salivary specific anti-HDS IgG and IgA in the group immunized by HDS-KLH was significantly higher than that in control group (P < 0.05). The Keyes caries score of GTF, HDS and HDS-KLH immunized group were significantly lower than that of control group, especially lower in smooth tooth surface.
CONCLUSIONArtificial antigen HDS-KLH could induce immune response. As a peptide vaccine, HDS-KLH could reduce the caries incidence in experimental rat model.
