Study of mutations in 5'-noncoding region of BCL-6 gene in B-cell non-Hodgkin's lymphomas.
- Author:
Da-Liu MIN
1
;
Xiao-Yan ZHOU
;
Wen-Tao YANG
;
Meng-Hong SUN
;
Hong-Fen LU
;
Tai-Ming ZHANG
;
Ai-Hua ZHENG
;
Pei-Zhen CAO
;
Da-Ren SHI
Author Information
- Publication Type:Journal Article
- MeSH: 5' Untranslated Regions; genetics; Adolescent; Adult; Aged; Aged, 80 and over; Base Sequence; Child; DNA-Binding Proteins; genetics; Female; Humans; Lymphoma, B-Cell; genetics; pathology; Lymphoma, Non-Hodgkin; genetics; pathology; Male; Middle Aged; Molecular Sequence Data; Point Mutation; Proto-Oncogene Proteins; genetics; Proto-Oncogene Proteins c-bcl-6; Transcription Factors; genetics
- From: Chinese Journal of Pathology 2004;33(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate BCL-6 gene mutations in B-cell non-Hodgkin lymphomas (B-NHL) and their implications in lymphoma pathogenesis.
METHODSPolymerase chain reaction (PCR) and direct DNA sequencing methods were used to identify mutations in the 5'-noncoding region of BCL-6 gene in 135 cases of B-NHL, 5 cases of T-NHL, 5 cases of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and 10 cases of reactive hyperplasia of lymph node.
RESULTSMutations were identified in 6 cases of nodal DLBCL (27.3%), 4 cases of FL (22.2%), 4 cases of MALT lymphoma (22.2%), 4 cases of extranodal DLBCL (20.7%) and 2 cases of LRH (20%). No mutations were detected in T-NHL and NLPHL (P < 0.05). There were no significant differences in incidences of BCL-6 gene mutations between nodal and extranodal DLBCL (P > 0.05). All mutations were base substitutions and the frequency of single-base change was 0.14 x 10(-2)/bp approximately 0.68 x 10(-2)/bp.
CONCLUSIONSMutations of the 5'non-coding region of BCL-6 gene may be involved in the pathogenesis and progression of B-NHL. Molecular demonstration of such mutations may provide a marker of lymphomas derived from the germinal center-related B cells.
