Growth suppression of colon cancer cells in vitro by DPC4 gene expression and its mechanism.
- Author:
Yang LIU
1
;
Ji-Fang WEN
;
Jing-He LI
;
De-Sheng XIAO
;
Zhong-Liang HU
;
Geng-Qiu LUO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma; genetics; metabolism; pathology; Cell Cycle Proteins; biosynthesis; genetics; Cell Division; Colorectal Neoplasms; genetics; metabolism; pathology; Cyclin-Dependent Kinase Inhibitor p21; DNA-Binding Proteins; biosynthesis; genetics; Gene Transfer Techniques; Genes, Tumor Suppressor; Humans; Smad4 Protein; Trans-Activators; biosynthesis; genetics; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Pathology 2004;33(3):247-250
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of DPC4 gene expression on the growth of colon cancer cells and its mechanism.
METHODSExpression plasmid pcDNA3.1-DPC4 was constructed and transfected into the colon cancer cell line SW620 by use of lipofectamine gene transfer technique. DPC4 protein expression was detected by Western blot and immunocytochemistry. The effect of DPC4 gene on the growth of SW620 cells was monitored by population doubling time (PDT) and cloning efficiency. The influence of DPC4 expression on p21WAF1 transcription was investigated by RT-PCR to detect p21WAF1 mRNA.
RESULTSSuccessful expression of DPC4 protein was detected in the transfected SW620 cells. Compared with the control cells, PDT (74 h) of the DPC4 expressing cells was prolonged and the cloning efficiency (21%) decreased. In addition, the mRNA level of p21(WAF1) in DPC4 transfected cells was increased.
CONCLUSIONSOverexpression of DPC4 gene inhibits the growth of colon cancer in vitro, and induction of p21(WAF1) expression may be an important functional aspect of DPC4.
