Detection of EWS-FLI1 fusion transcript in Ewing's sarcoma/peripheral primitive neuroectodermal tumors by one-step RT-PCR using paraffin-embedded tissues.
- Author:
Feng LI
1
;
Bin CHANG
;
Xin-xia LI
;
Li-juan PANG
;
Hong-fen LU
;
Jian WANG
;
Meng-hong SUN
;
Da-ren SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Diagnosis, Differential; Female; Humans; Male; Middle Aged; Neuroectodermal Tumors, Primitive, Peripheral; diagnosis; metabolism; Oncogene Proteins, Fusion; metabolism; Paraffin Embedding; Proto-Oncogene Protein c-fli-1; RNA-Binding Protein EWS; Reverse Transcriptase Polymerase Chain Reaction; Rhabdomyosarcoma; diagnosis; metabolism; Sarcoma, Ewing; diagnosis; metabolism; Sarcoma, Synovial; diagnosis; metabolism; Transcription Factors; metabolism
- From: Chinese Journal of Pathology 2004;33(4):328-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of EWS-FLI1/ERG fusion transcript resulting from t(11;12)(q24;12) in paraffin-embedded tissues and its diagnostic implication for Ewing's sarcoma/peripheral primitive neuroectodermal tumors (ES/pPNET).
METHODSOne-step reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect a characteristic EWS-FLI1/ERG fusion transcript in 25 cases of ES/pPNET and 15 cases of other small round cell tumors (including 8 cases of rhabdomyosarcoma, 4 cases of synovial sarcoma, 2 cases of neuroblastoma and 1 case of lymphoma) using formalin-fixed and paraffin-embedded tissues.
RESULTSEWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%). The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript.
CONCLUSIONSDetection of EWS-FLI1/ERG fusion transcript is a reliable index for molecular diagnosis of ES/pPNET. One-step RT-PCR is a practical method for such analysis in routine paraffin-embedded tumor tissues.
