Expression and significance of DARPP-32 in gastric carcinoma.
- Author:
Jin WANG
1
;
Yang-lin PAN
;
Na LIU
;
Chang-cun GUO
;
Liu HONG
;
Dai-ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; Aged; Antibiotics, Antineoplastic; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Cell Line, Tumor; Dopamine and cAMP-Regulated Phosphoprotein 32; Doxorubicin; pharmacology; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Female; Gastric Mucosa; metabolism; Humans; Male; Middle Aged; Nerve Tissue Proteins; metabolism; Phosphoproteins; metabolism; Stomach Neoplasms; metabolism; Vincristine; pharmacology
- From: Chinese Journal of Pathology 2004;33(4):350-353
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the significance of DARPP-32 protein expression in gastric carcinoma tissue and cell lines.
METHODSThe expression of DARPP-32 protein in normal gastric mucosa and gastric carcinoma tissue was evaluated by immunohistochemical staining using streptavidin-biotin complex technique. The expression in gastric carcinoma tissue and cell lines was evaluated by Western blotting.
RESULTSThe expression rate of DARPP-32 protein in gastric adenocarcinoma tissue (92.7%) was significantly higher than that in normal gastric mucosa (52.6%, P < 0.05). There was no significant association between DARPP-32 protein expression and degree of tumor differentiation, local invasion and distant metastasis. As compared with adjacent non-carcinomatous gastric mucosa, both DARPP-32 and its truncated isoform t-DARPP were overexpressed in gastric adenocarcinoma tissue (t = 2.45, P = 0.015); and t-DARPP overexpression was more frequently seen. Expression of DARPP-32 and t-DARPP could also be detected in human gastric cancer cell lines. The expression of DARPP-32 protein was obviously reduced in SGC7901 drug-resistant cell strains.
CONCLUSIONSDARPP-32 is overexpressed in gastric carcinoma. It may play an important role in gastric carcinogenesis. The underlying signal pathways in neoplastic gastric epithelium may also be related to the multi-drug resistance property of gastric cancer cells.
