The role of Egr-1 and NF-kappaB in the pathogenesis of silicosis: an in-vitro study.
- Author:
Hai-yan NIU
1
;
Qing-fu ZENG
;
Xiang LI
;
Ling CHU
;
Yong-bin HU
;
Jin-sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies; immunology; Cells, Cultured; DNA-Binding Proteins; genetics; immunology; Early Growth Response Protein 1; Immediate-Early Proteins; genetics; immunology; Macrophages; cytology; metabolism; Mice; NF-kappa B; genetics; immunology; Oligonucleotides, Antisense; pharmacology; RNA, Messenger; biosynthesis; genetics; Silicon Dioxide; pharmacology; Silicosis; etiology; Transcription Factors; genetics; immunology; Transforming Growth Factor beta; biosynthesis; genetics; Transforming Growth Factor beta1; Tumor Necrosis Factor-alpha; biosynthesis; genetics
- From: Chinese Journal of Pathology 2004;33(4):363-367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the correlation between the expression of Egr-1 and NF-kappaB and the up-regulation of TNF-alpha and TGF-beta1 in macrophages after stimulation by silica in-vitro.
METHODSMacrophages were treated with antibodies against Egr-1 and NF-kappaB and antisense oligonucleotides. The level of TNF-alpha protein in the cell supernatant was then measured using enzyme-linked immunoadsorbent assay (ELISA). The expression of TGF-beta1 protein was detected by immunocytochemistry. The expression of TNF-alpha and TGF-beta1 mRNAs was also monitored by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSCompared with silica-stimulated macrophages untreated with antibodies, the cells treated with 10 micro g/ml of Egr-1 or NF-kappaB antibodies were associated with reduced expression of TNF-alpha and TGF-beta1 proteins and mRNAs (P < 0.05). Compared with silica-stimulated untransfected group, the antisense group was associated with obvious reduction in the expression of TNF-alpha and TGF-beta1 proteins and mRNAs (P < 0.05).
CONCLUSIONThe expression of TNF-alpha and TGF-beta1 mRNAs and proteins are associated with activation of Egr-1 and NF-kappaB in macrophages, after stimulation by silica. It is possible that the corresponding antibodies and antisense oligonucleotides may become a potential therapeutic tool in the management of silicosis in the future.
