Assessment of P504S immunohistochemistry in diagnosis and differential diagnosis of prostatic adenocarcinoma.
- Author:
Guang-yong CHEN
1
;
Li-na LIU
;
Xiao-ge ZHOU
;
Chang-huai ZHANG
;
Shou-fang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; diagnosis; DNA-Binding Proteins; Diagnosis, Differential; Genes, Tumor Suppressor; Humans; Immunohistochemistry; Keratins; analysis; Male; Phosphoproteins; analysis; Precancerous Conditions; diagnosis; Prostate; enzymology; Prostatic Hyperplasia; diagnosis; Prostatic Intraepithelial Neoplasia; diagnosis; Prostatic Neoplasms; diagnosis; Racemases and Epimerases; analysis; Trans-Activators; analysis; Transcription Factors; Tumor Suppressor Proteins
- From: Chinese Journal of Pathology 2004;33(5):419-423
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the utility of P504S immunohistochemistry in the diagnosis and differential diagnosis of prostatic adenocarcinoma.
METHODSLight microscopy and immunohistochemistry examinations (EnVision staining) were performed in 117 cases of prostatic adenocarcinoma, PIN, AAH, ASAP, BPH and normal prostatic tissue to correlate the morphology and protein expression of P504S, 34betaE12, and P63.
RESULTSSeventy-one of the 78 (91%) cases of prostatic adenocarcinoma stained positive for P504S, with strong cytoplasmic granular staining in most cases, and a weak or intense granular staining along the circumferential luminal and apical cell border membrane in a few cases. Negative P504S immunostaining was observed in 7 of 78 (9%) cases of prostatic adenocarcinoma, all of which were clear cell type prostatic adenocarcinoma. Cases of PIN (9 cases), AAH (6 cases) and ASAP (2 cases) showed various expression levels of P504S. Sixty-five of 68 (96%) cases of normal prostates and BPH were negative for P504S and basal cell hyperplasia cases were also negative.
CONCLUSIONSP504S is a useful marker for microscopic diagnosis of prostatic adenocarcinoma, and immunohistochemistry study using a combination of P504S and 34betaE12/p63 may be of greater benefit in aiding the differential diagnoses.
