mtDNA mutations in mouse tumors.
- Author:
Ji-gang DAI
1
;
Jia-xin MIN
;
Guo-qiang ZHANG
;
Hong WEI
;
Ying-bin XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; DNA, Mitochondrial; genetics; DNA, Neoplasm; genetics; Electron Transport Complex I; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mutation; Neoplasms, Experimental; genetics; pathology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Proteins; genetics; RNA, Transfer, Glu; genetics; RNA, Transfer, Ile; genetics; RNA, Transfer, Met; genetics
- From: Chinese Journal of Pathology 2004;33(5):458-461
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate variations of mtDNA in mouse tumors and to explore the relationship between mtDNA mutations and murine carcinogenesis.
METHODSVariations of D-loop, ND3 and tRNAIle + Glu + Met gene fragments of mtDNA from six mouse tumor cell lines were analyzed by PCR-RFLP and PCR-SSCP techniques.
RESULTSND3 and tRNAIle + Glu + Met gene fragments of mtDNA from the tumors showed no variations at 27 endonuclease sites. The D-loop of mtDNA from Hca-F demonstrated an additional endonuclease site of Hinf I in contrast to the inbred mouse. Upon PCR-SSCP analysis, the D-loop of mtDNA was found to possess mutations in 4 of 6 tumors.
CONCLUSIOND-loop appears to be the hot spot for tumor mtDNA mutations, which may contribute to the carcinogenesis of murine tumors.
