Detection of Y chromosome loss by fluorescence in situ hybridization in pancreatic cancer.

Xuan Zeng; Da-chun Zhao; Tong-hua Liu; Sha-fei WU; Jie Gao

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Detection of Y chromosome loss by fluorescence in situ hybridization in pancreatic cancer.

Author: Xuan ZENG ¹ ; Da-Chun ZHAO ; Tong-Hua LIU ; Sha-Fei WU ; Jie GAO
Author Information

1. Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China.
Publication Type:Journal Article
MeSH: Adenocarcinoma; genetics; Aged; Biomarkers, Tumor; Chromosome Deletion; Chromosomes, Human, Y; Humans; In Situ Hybridization, Fluorescence; Male; Middle Aged; Pancreatic Neoplasms; genetics
From: Chinese Journal of Pathology 2004;33(6):523-526
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the correlation between loss of Y chromosome and development of pancreatic cancer.
METHODThe status of Y chromosome was analyzed by two color interphase fluorescence in situ hybridization. performed on paraffin sections of pancreatic cancer tissues from 15 Chinese males. The probes located on the heterochromatin region of chromosome Y (maps to Yq12) and on the alpha satellite of X chromosome (maps to centromeric region) were selected for testing and as control respectively.
RESULTSThe cancer cells from 10 out of the 15 pancreatic cancer patients studied showed loss of chromosome Y. The Y chromosome in the cells of surrounding non-neoplastic pancreatic tissues was intact.
CONCLUSIONSLoss of chromosome Y occurs non-randomly in tumor cells of Chinese male patients with pancreatic cancer. This cytogenetic aberration, which happens in high frequency, may serve as one of the markers for pancreatic malignancy.